In order to understand the status of fishery resources after the implementation of conservation management and stocking of the Chishui River,and to provide a data base for future fish conservation and stocking work,this study was conducted in 2016 by using three methods of fluorescent tagging,dorsal fin T-tagging,and fin clipping tagging to mark and recapture S.grahami as an indicator species,and using breeding experiments to deduce the marker loss rate.Three samples were taken in May,June and July to assess the fish resources in the source section of the Chishui River.The results showed that the instantaneous resource numbers of S.grahami in the source section of the Chishui River during the assessment period were 1.99×10⁴,2.12×10⁴ and 1.68×10⁴ individuals,respectively;the instantaneous biomass was 467.6 kg,726.1 kg and 909.3 kg,respectively.Both the clipped fin marker group and the dorsal fin T marker group suffered from fall off and loss,with the loss rate of the dorsal fin T marker increasing exponentially with the increase of breeding time.The evaluation results showed that the continuous proliferation and release had a significant effect on the stability of fish resources in the headwaters of Chishui River and was conducive to the recovery of fish resources.